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为研究褪黑素对低氧引起大鼠大脑皮层脑片氨基酸释放变化的影响， 利用反相高效液相色谱结合荧光检测法， 测定了孵育液中氨基酸类神经递质的含量。低氧条件为通入９１－６％ Ｎ２ 和８－４ ％ Ｏ２ 的混合气体。低氧３０ ｍｉｎ 时， 大鼠大脑皮层脑片孵育液中， 氨基酸类神经递质天冬氨酸、谷氨酸、谷氨酰胺、甘氨酸、牛磺酸和γ氨基丁酸的含量显著增加， 其含量分别是正常氧组的２４０－４ ％ ，３３４－３ ％ ，２００－６ ％ ，２１０－４ ％ ，１６８－６ ％ 和２６３－９ ％ ， 与正常氧组比较Ｐ＜ ０－０１ 。予以褪黑素可以显著降低兴奋性氨基酸的释放， 其中天冬氨酸和谷氨酸的含量分别是低氧组的５５－１ ％ 和５７－０ ％ 。结果表明， 褪黑素具有降低低氧所致的脑片释放兴奋性氨基酸的作用。
In order to study the effect of melatonin on the release of amino acids in cerebral cortex of rats induced by hypoxia, the content of amino acid neurotransmitters in the incubation solution was determined by RP-HPLC with fluorescence detection. Hypoxic conditions for the introduction of 91-6% N2 and 8-4% O2 mixed gas. At 30 min of hypoxia, the contents of amino acid neurotransmitters aspartate, glutamate, glutamine, glycine, taurine and γaminobutyric acid increased significantly in the cerebral cortex brain slices of rats, The contents were 240-4%, 334-3%, 200-6%, 210-4%, 168-6% and 263-9% of the normal oxygen group respectively, P <0-01 compared with the normal oxygen group. Melatonin treatment significantly reduced the release of excitatory amino acids, with aspartate and glutamate levels of 55-1% and 57-0%, respectively, in hypoxia group. The results showed that melatonin has the effect of reducing the release of excitatory amino acids in brain slices caused by hypoxia.