目的研究黏附因子丛生因子A(clumping factor A,ClfA)在金黄色葡萄球菌(SAU)不同菌株中的分布情况,为开发具有广泛保护效果的SAU疫苗奠定基础。方法采用PCR法扩增目的基因,构建重组质粒,以大肠杆菌BL21(DE3)为表达宿主,对目的蛋白进行表达、纯化。PCR及点杂交法检测ClfA在不同菌株中的分布情况。结果得到纯度较高的目的蛋白,PCR及点杂交结果显示ClfA存在于菌株USA300,而在菌株546、1884、1885中并无分布。结论 ClfA在SAU各菌株中分布不广泛,可能难以作为具有广泛保护效果的SAU疫苗组分。 Objective To study the distribution of clumping factor A (ClfA) in different strains of Staphylococcus aureus (SAU) and lay the foundation for the development of SAU vaccine with broad protective effect. Methods The target gene was amplified by PCR and the recombinant plasmid was constructed. The target protein was expressed and purified by using E. coli BL21 (DE3) as host. PCR and dot blot hybridization method to detect the distribution of ClfA in different strains. As a result, the target protein with higher purity was obtained. PCR and dot blot showed that ClfA was present in strain USA300, but not in strain 546,1884,1885. Conclusion ClfA is not widely distributed in SAU strains and may be difficult to use as a SAU vaccine component with a broad range of protective effects.