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为揭示小麦幼苗在Fe或Cu胁迫下的抗氧化反应机制,以宁春4号小麦为材料,比较了不同浓度(0,100,300,500μmol/L)Fe Cl3或Cu Cl2处理下幼苗根羟自由基(·OH)含量、抗氧化酶活性和多胺降解酶活性的变化.结果显示:Fe或Cu处理诱导小麦根·OH含量升高,且高浓度Cu处理下样品·OH含量高于相同浓度Fe处理的样品的.Fe或Cu处理对各种抗氧化酶活性有不同的影响:Fe诱导超氧化物歧化酶活性升高,而Cu抑制该酶活性;Fe处理不影响过氧化物酶活性,而Cu诱导该酶活性升高;过氧化氢酶活性在Fe或Cu处理下均受到明显的抑制;Fe或Cu处理抑制谷胱甘肽还原酶活性;300,500μmol/L的Fe和100,300μmol/L的Cu诱导抗坏血酸过氧化物酶活性的升高.此外,不同浓度的Fe或Cu对多胺氧化酶(PAO)、二胺氧化酶(DAO)和细胞壁过氧化物酶(cw-POD)活性均有明显的抑制作用.结果表明:Fe或Cu胁迫诱导对小麦根不同抗氧化酶产生不同的影响,最终导致小麦根·OH积累;PAO,DAO和cw-POD可能不参与Fe或Cu胁迫诱导的ROS积累.
To reveal antioxidant mechanisms of wheat seedlings under Fe or Cu stress, the effects of different concentrations (0, 100, 300 and 500μmol / L) of FeCl3 or CuCl2 on the root · OH radical ) Content, antioxidant enzyme activity and polyamine degrading enzyme activity.The results showed that the root · OH content of wheat increased with Fe or Cu treatment, and · OH content of high concentration Cu treatment was higher than that of the same concentration of Fe treatment Fe or Cu treatment had different effects on the activities of various antioxidant enzymes: Fe induced superoxide dismutase activity increased, while Cu inhibited the enzyme activity; Fe treatment did not affect peroxidase activity, while Cu induced the The activity of catalase was significantly inhibited under the treatment of Fe or Cu; the activity of glutathione reductase was inhibited by Fe or Cu treatment; the effect of 300,500μmol / L Fe and 100,300μmol / L Cu induced ascorbic acid Peroxidase activity.In addition, different concentrations of Fe or Cu significantly inhibited the activity of polyamine oxidase (PAO), diamine oxidase (DAO) and cell wall peroxidase (cw-POD) The results showed that: Fe or Cu stress induced different antioxidant enzymes in wheat roots Impact, eventually leading to the accumulation of wheat roots · OH; PAO, DAO and cw-POD Fe or Cu may not be involved in stress-induced ROS accumulation.