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本文介绍了一种简单的一步转化法,在猪胰岛素的B_(30)位,通过胰蛋白酶的转酰胺作用,变为人胰岛素,并通过硅胶层析完成产品的纯化。为了研究胰蛋白酶的专一性和它的转酰胺机制已制备了胰岛素B_(30)的类似物。
This article describes a simple one-step transformation method that converts human insulin at the B_ (30) position of porcine insulin by transamidation of trypsin and purifies the product by silica gel chromatography. In order to study the specificity of trypsin and its transamidation mechanism, an analog of insulin B_ (30) has been prepared.