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近年,支气管哮喘与变应性鼻炎等变应性疾患增加,其主要原因可能由于室内灰尘中螨的增加,即由于室内条件的改变,形成了螨繁殖的良好环境。以前通过收集清扫机内的灰尘测定室内灰尘中的螨,通常在显微镜下计数,但这种方法需要特殊的技术,且费时费力。螨体及其产物中多含有鸟嘌呤,有必要研究螨体内含有的鸟嘌呤量与室内灰尘中螨数相关的可能性。鸟嘌呤测定用液相色谱法(日立L-4200型)、UV-VIS检测器、开柱(655A-25)、D-2500型色谱数据处理机及硅胶柱)NO3013-N,4mm×150mm)。洗脱液A:0.1mol/L醋酸
In recent years, allergic diseases such as bronchial asthma and allergic rhinitis have been increased. The main reason may be due to the increase of mites in indoor dust, that is, a good environment for mite breeding due to the change of indoor conditions. Previously, mites in indoor dust were measured by collecting dust in the sweeping machine and were usually counted under a microscope. However, this method requires special techniques and takes time and effort. It is necessary to study the possibility that the amount of guanine contained in the mite body is related to the number of mites in the indoor dust. The guanine was measured by liquid chromatography (Hitachi L-4200), UV-VIS detector, open column (655A-25), D-2500 chromatographic data processor and silica gel column NO3013-N, 4 mm × 150 mm) . Eluent A: 0.1 mol / L acetic acid