论文部分内容阅读
目的 探讨ｐ５３ 、ｐ１６ 、 Ｃｙｃｌｉｎ Ｄ１ 表达与胃癌细胞增生状况的关系。方法 采用免疫组化 Ａ Ｂ Ｃ 法检测５８ 例胃癌中ｐ５３ 、ｐ１６ 、 Ｃｙｃｌｉｎ Ｄ１ 和 Ｐ Ｃ Ｎ Ａ 的表达状况，并对 Ｐ Ｃ Ｎ Ａ 免疫阳性细胞作半定量分析。结果 ｐ５３ 、ｐ１６ 、 Ｃｙｃｌｉｎ Ｄ１ 阳性表达率分别为５１ ．７ ％ （３０／５８） 、４８ ．３ ％ （２８／５８） 、５３ ．４ ％ （３１／５８） ；ｐ５３ 、 Ｃｙｃｌｉｎ Ｄ１ 阳性组织中，单位面积 Ｐ Ｃ Ｎ Ａ 阳性细胞数分别高于ｐ５３ 、 Ｃｙｃｌｉｎ Ｄ１ 阴性组织的 Ｐ Ｃ Ｎ Ａ 阳性细胞数（ Ｐ＜ ０ ．０５） ；ｐ１６ 阴性组织的阳性 Ｐ Ｃ Ｎ Ａ 细胞高于阳性ｐ１６ 组织，但差异无显著性（ Ｐ＞ ０ ．０５） 。结论 ｐ５３ 、 Ｃｙｃｌｉｎ Ｄ１ 高表达可能是胃癌细胞增生活跃的重要影响因素。
Objective To investigate the relationship between the expression of p53, p16 and Cyclin D1 and the proliferation of gastric cancer. Methods Immunohistochemical A B C method was used to detect the expression of p53, p16, Cyclin D1 and PCNA in 58 cases of gastric cancer. Semi-quantitative analysis of P C N A immunopositive cells was performed. Results The positive expression rates of p53, p16 and Cyclin D1 were 51. 7 % (30/58), 48. 3% (28/58), 53. In 4% (31/58); p53 and Cyclin D1 positive tissues, the number of P C N A positive cells per unit area was higher than that of p C N A positive cells in p53 and Cyclin D1 negative tissues (P < 0.05). The positive P C N A cells in p16-negative tissue were higher than those in positive p16 tissue, but the difference was not significant (P > 0.05). Conclusion The high expression of p53 and Cyclin D1 may be an important factor in the proliferation of gastric cancer cells.