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用双脱氧链终止法分析了克隆的番木瓜环斑病毒（PRV）Ys株系外壳蛋白（CP）基因的序列，结果表明YsCP基因全长858nt。对PRV国内外16个株系或分离物CP基因的比较发现，YsCP基因与国内株系或分离物CP基因的同源性较高（94．44％～97．68％），而与国外株系或分离物CP基因的同源性较低（88．88％～92．70％）。CP基因之间的差异主要靠近基因5’端，特别是在YSCP基因第63nt后连续缺失6nt，SmGTHAI和SRI的CP基因也在此处缺失3nt。将YsCP基因插入中间质粒pRokⅡ的CaMV35S启动于和nos终止序列之间形成CP基因的植物表达载体pRPCY，通过三亲交配使pRPCY进入农杆菌LBA4404，与其中的pAL4404构成双元载体系统。
The sequence of the coat protein (CP) gene of the cloned papaya ring spot virus (YV) strain Ys was analyzed by dideoxy chain termination method. The results showed that the YsCP gene was 858 nt in length. Comparison of the CP genes of 16 strains or isolates of PRV at home and abroad revealed that the homology of the YsCP gene with the CP gene of domestic strains or isolates was high (94.44% -97.68%), while that of foreign strains The homology of the CP gene between isolate and isolate was low (88.88% -92.70%). The difference between the CP genes is mainly close to the 5 ’end of the gene, and in particular, 6 nt are continuously deleted after 63 nt of the YSCP gene, and the CP gene of SmGTHAI and SRI is also deleted here by 3 nt. The YsCP gene was inserted into the middle plasmid pRokII CaMV35S to initiate the plant expression vector pRPCY which forms the CP gene with the termination sequence of nos. The pRPCY was introduced into Agrobacterium LBA4404 through the triparental mating and the binary vector system was constructed with pAL4404.