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目的建立超高效液相色谱法测定米豆腐中偶氮甲酰胺含量。方法使用超高效液相色谱法进行测定,色谱条件为:色谱柱:Waters C_(18)(150 mm×2.1 mm,5μm),流动相:水-甲醇(90:10,V:V),流速:0.3 m L/min,检测波长:275 nm,柱温:30℃。结果偶氮甲酰胺的色谱峰形对称,并能与其他物质完全分离;在14.98~149.8 ng范围内线性关系良好,相关系数r=0.9991(n=6);方法检出限为0.5μg/g,方法定量限为1.6μg/g,平均加标回收率为98.0%,相对标准偏差3.5%(n=3)。结论该方法前处理简单、快速,定量准确,灵敏度高,可用于检测米豆腐中偶氮甲酰胺含量。
Objective To establish a method for the determination of azodicarbonamide in rice tofu by ultra performance liquid chromatography. The method was carried out using ultra performance liquid chromatography with the following chromatographic conditions: Waters C 18 (150 mm × 2.1 mm, 5 μm), mobile phase of water-methanol (90:10, V: V) : 0.3 m L / min, detection wavelength: 275 nm, column temperature: 30 ℃. Results The azodicarbonamide peak was symmetrical and completely separated from other substances. The linear range was 14.98-149.8 ng with a correlation coefficient of 0.9991 (n = 6). The detection limit was 0.5 μg / g The limit of quantification was 1.6 μg / g. The average recovery was 98.0% and the relative standard deviation was 3.5% (n = 3). Conclusion The pretreatment method is simple, rapid, accurate and sensitive. It can be used to detect azodicarbonamide content in rice tofu.