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[目的]氮肥在农业中占有重要的地位,因此植物氮素的营养学研究日益受到重视。了解稳定性~(15)N示踪技术应用于氮素的分配、积累及氮素利用率情况。了解稳定性~(15)N示踪技术在农业氮肥利用中的应用,为提高氮肥利用率、实现生态农业可持续发展提供必要的理论支持。[方法]利用文献资料概述稳定性~(15)N示踪技术在小麦、水稻、烟草、果树、豆科作物上的应用,着重论述氮素吸收、利用、分配的研究现状。[结果]稳定性~(15)N示踪技术是研究氮素营养吸收规律的一种方便、快捷、灵活的方法之一,在科学施肥、植物营养、农用化学物质残留等农业方面已被广泛应用。[结论]今后,应将稳定性~(15)N示踪技术应将更多的应用到农业、环境、生物食品学领域,拓宽稳定性同位素的应用范围,并建立和完善相关的同位素定量化解析模型,提高测定位同位素的准确性。
[Objective] Nitrogen fertilizer occupies an important position in agriculture, so the nutrition research of plant nitrogen has been paid more and more attention. Understand the stability of ~ (15) N tracer technology used in nitrogen distribution, accumulation and nitrogen utilization. To understand the application of stable ~ (15) N tracer technology in agricultural nitrogen utilization, provide the necessary theoretical support for increasing the utilization rate of nitrogen fertilizer and realizing the sustainable development of ecological agriculture. [Method] The application of stable ~ (15) N tracer technology in wheat, rice, tobacco, fruit tree and leguminous crops was summarized in the literature. The research status of nitrogen absorption, utilization and distribution was mainly discussed. [Result] The stable ~ (15) N tracer technology was one of the convenient, fast and flexible methods to study the law of nitrogen nutrient absorption. It has been widely used in agriculture such as scientific fertilization, plant nutrition and agricultural chemical residue application. [Conclusion] In the future, the stable ~ (15) N tracing technique should be more applied to the field of agriculture, environment and bio-food science, broaden the application range of stable isotopes, and establish and improve related isotope quantification Analyze the model to improve the accuracy of the measured isotopes.