【目的】以金针菇全基因组测序数据为基础研究其L-赖氨酸的从头合成途径及其关键基因。【方法】采用Illumina Hiseq2000和Roche454 FLX+两种方法完成金针菇单孢菌株Dan3的基因组测序,基于全基因组序列筛选金针菇中赖氨酸生物合成的关键基因,并分析这些基因编码的蛋白质基本理化性质;预测其亚细胞定位情况及蛋白的二级结构。【结果】金针菇Dan3全基因组序列长度为34.17 Mb,预测到8个参与α-氨基己二酸途径的关键基因,这些基因都含有多个内含子和外显子,二级结构主要由α-螺旋和无规则卷曲组成,有3个蛋白定位于线粒体。【结论】金针菇是通过α-氨基己二酸途径合成赖氨酸,基因组中预测到与该途径相关的几乎所有的酶。 【Objective】 The purpose of this study was to study the de novo synthesis pathway of L-lysine and its key genes based on the whole genome sequencing data of Flammulina velutipes. 【Method】 The genomic sequence of Dan3 strain of Flammundia tenuiflora was determined by Illumina Hiseq2000 and Roche454 FLX +. The key genes of Lysine biosynthesis in Flammulina velutipes were screened based on whole genome sequence. The basic physical and chemical properties of the protein encoded by these genes were analyzed. Its subcellular localization and protein secondary structure. 【Result】 The whole genome sequence of Flammulina velutipes Dan3 was 34.17 Mb. Eight key genes involved in the α-aminoadipic acid pathway were predicted. These genes all contained multiple introns and exons. The secondary structure was mainly composed of α- Spiral and random curl composition, there are three proteins located in mitochondria. 【Conclusion】 Flammulina velutipes synthesize lysine through the α-aminoadipic acid pathway, and almost all enzymes predicted in the genome are related to this pathway.