用神经元特异性烯醇酶的抗血清进行PAP免疫组织化学染色(简称抗NSEPAP染色),观察体外培养大鼠小脑颗粒细胞的迁移,分化与成熟。发现随着细胞从种植片向周围迁移,突起逐渐形成,抗NSE-PAP染色反应于第四天从阴性转为阳性,染色逐渐增强,以10～12d最为明显,并由胞体延及突起。提示体外培养颗粒细胞的分化和成熟与体内的发育过程相似,可为体外研究神经细胞的发育提供较理想的实验模型。 PAP immunohistochemical staining (anti-NSEPAP staining) was performed using antisera of neuron-specific enolase and the migration, differentiation and maturation of cultured rat cerebellar granule cells were observed. It was found that as the cells migrated from the implant to the periphery, the protrusions gradually formed. The anti-NSE-PAP staining reaction turned from negative to positive on the fourth day. The staining gradually increased to the most obvious at 10 to 12 days and was prolonged by the cell body. These results suggest that the differentiation and maturation of cultured granulosa cells in vitro are similar to those in vivo, which may provide an ideal experimental model for studying the development of nerve cells in vitro.