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目的观察壳聚糖抗菌成膜喷剂杀(抑)菌效果。方法采用抑菌环试验和GB15979-2002规定的载体杀菌试验方法,对该抗菌成膜喷剂体外的杀(抑)菌效果进行评价。结果该壳聚糖抗菌成膜喷剂为无色透明液体,其原液含壳聚糖约15 g/L。该喷剂原液对大肠杆菌、金黄色葡萄球菌和白色念珠菌的抑菌环直径分别为16.88 mm、19.71 mm和10.26 mm。以该喷剂原液对金黄色葡萄球菌、大肠杆菌和白色念珠菌作用20 min,平均杀菌率均达99.99%。结论该壳聚糖抗菌成膜喷剂对细菌繁殖体和致病酵母菌均具有较强的杀(抑)菌效果。
Objective To observe the killing effect of chitosan antibacterial film-forming spray. Methods The bacteriostasis test and the bacteriostatic test method of GB15979-2002 were used to evaluate the bactericidal effect of the antibacterial film-forming sprays in vitro. Results The chitosan antibacterial film-forming spray was a colorless and transparent liquid containing about 15 g / L chitosan as its stock solution. The antibacterial rings of Escherichia coli, Staphylococcus aureus and Candida albicans were 16.88 mm, 19.71 mm and 10.26 mm, respectively. The original stock solution of Staphylococcus aureus, Escherichia coli and Candida albicans for 20 min, the average sterilization rate reached 99.99%. Conclusion The chitosan antibacterial film-forming sprays have strong bactericidal and bacteriostatic effects on both bacterial and pathogenic yeasts.