论文部分内容阅读
目的 探讨抗聚角蛋白微丝蛋白抗体 (AFA)对RA诊断的意义 ,并比较其与抗核周因子抗体 (APF) ,抗角蛋白抗体 (AKA)以及HLA DR4之间的相关性。方法 用免疫印迹法 (WB)检测 15 7例类风湿关节炎 (RA)血清标本和 10 4例对照血清 ,包括系统性红斑狼疮 (SLE)、干燥综合征(SS)、骨关节炎 (OA)等的AFA。APF和AKA用间接免疫荧光法检测。结果 15 7例RA病人的AFA敏感性和特异性分别为 36 3% ,93 4% ,与疾病对照组和正常对照组相比 ,差异有显著性 (P <0 0 5 )。 5 7例AFA阳性的血清中 ,其中AKA同时阳性的有 36例 ,重叠率为 6 3 2 % ;APF同时阳性的有 38例 ,重叠率为 6 6 7% ;HLA DR4同时阳性的有 30例 ,重叠率为 5 2 6 %。统计学分析证实AFA与AKA、APF及HLA DR4之间有相关性。结论 AFA对RA具有很高的特异性 ,可用于RA的临床诊断。AFA与APF、AKA及HLA DR4有相关性。AFA不能完全替代APF和AKA ,三项临床检测可互相补充 ,提高RA的诊断率
Objective To investigate the diagnostic value of anti-Keratin filaggrin antibody (AFA) in RA and compare the correlation with anti-nuclear factor antibody (APF), anti-keratin antibody (AKA) and HLA DR4. Methods Fifteen sera of rheumatoid arthritis (RA) and 104 control sera were detected by Western blotting, including systemic lupus erythematosus (SLE), Sjogren ’s syndrome (SS), osteoarthritis (OA) Etc. AFA. APF and AKA were detected by indirect immunofluorescence. Results The AFA sensitivity and specificity of 15 7 RA patients were 36 3% and 93 4%, respectively, which were significantly different from those of the control group and the control group (P 0.05). Of 57 AFA-positive sera, 36 cases were positive for AKA at the same time, the overlap rate was 6 3 2%; 38 cases were APF positive at the same time, the overlap rate was 6 6 7%; 30 cases were HLA-DR 4 positive at the same time , Overlapping rate of 526%. Statistical analysis confirmed the correlation between AFA and AKA, APF and HLA DR4. Conclusion AFA is highly specific to RA and can be used in the clinical diagnosis of RA. AFA is associated with APF, AKA and HLA DR4. AFA can not completely replace APF and AKA, three clinical tests can complement each other to improve the diagnostic rate of RA