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目的研究系统性红斑狼疮(SLE)患者外周血CD1的表达与疾病活动性之间的关系。方法用流式细胞仪检测了47例SLE患者外周血单个核细胞CD1c及CD1d的表达及淋巴细胞亚群百分数。结果SLE活动组病人CD1c+及CD1d+细胞百分率显著增高(P<0.05),CD4+细胞百分率显著降低(P<0.01),CD3+、CD8+细胞百分率正常,CD20+细胞数增高(P<0.01)。稳定期病人CD1c+及CD1d+细胞百分率正常,CD4+、CD8+、CD20+细胞百分率均正常。SLE患者CD1c+、CD1d+细胞阳性率与患者SLEDAI的评分有显著的相关性(r=0.68与r=0.66,P<0.01),与抗dsDNA抗体的表达有显著相关性(r=0.36与r=0.41,P<0.05);SLE患者CD1c+细胞阳性率与抗磷脂抗体(ACA)的表达有显著的相关性(r=0.68,P<0.01),与血清C3水平有显著相关性(r=-0.35,P<0.05)。经治疗后CD1c及CD1d的表达明显下降。结论系统性红斑狼疮患者外周血CD1c与CD1d的表达与疾病的活动性明显相关,CD1c、CD1d可能在SLE脂类抗原及核酸类抗原的递呈及抗dsDNA抗体、抗磷脂抗体的产生中起重要作用。
Objective To study the relationship between CD1 expression and disease activity in peripheral blood of patients with systemic lupus erythematosus (SLE). Methods The expression of CD1c and CD1d in peripheral blood mononuclear cells and the percentage of lymphocyte subsets in 47 SLE patients were detected by flow cytometry. Results The percentage of CD1c + and CD1d + cells in SLE patients was significantly increased (P <0.05), the percentage of CD4 + cells was significantly decreased (P <0.01), the percentage of CD3 + and CD8 + cells was normal and the number of CD20 + cells was increased (P <0.01). Stable patients with normal percentage of CD1c + and CD1d + cells, CD4 +, CD8 +, CD20 + cells were normal. The positive rates of CD1c + and CD1d + cells in SLE patients were significantly correlated with the scores of SLEDAI patients (r = 0.68 and r = 0.66, P <0.01), and significantly correlated with the expression of anti-dsDNA antibodies , P <0.05). The positive rate of CD1c + cells in SLE patients was significantly correlated with the level of anti-phospholipid antibody (rA = 0.68, P <0.01) P <0.05). After treatment, the expression of CD1c and CD1d decreased significantly. Conclusions The expression of CD1c and CD1d in peripheral blood of patients with systemic lupus erythematosus is significantly correlated with the activity of the disease. CD1c and CD1d may play an important role in the presentation of SLE lipids and nucleic acid antigens and the production of anti-dsDNA and antiphospholipid antibodies effect.