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目的对经水作用的血样本DNA分型检验结果进行分析探讨。方法全血样本分为两组,水稀释组用水将全血样本稀释5、10、20、25、30倍后制作血斑;洗涤组分为纯水手洗、肥皂弱洗、肥皂强洗、84消毒液浸洗和洗衣粉机洗等5种洗涤方式。所有样本用IQ试剂盒提取DNA,Identifiler PlusTM试剂盒扩增,并进行分型检测。结果血液稀释组中心部位检材,均无等位基因丢失,除30倍稀释样本外,峰高均衡性均大于70%;外周部位检材出现2～10个等位基因丢失,峰高均衡性均小于50%。洗涤组中除84消毒液洗涤样本未检出DNA谱带外,其余均无等位基因丢失,而峰高及均衡性以手洗和肥皂弱洗样本更好。结论经水稀释或洗涤剂清洗的血样本,即使联苯胺预实验结果为阴性,选取合适的检验部位,仍可获得DNA分型。
Objective To analyze and discuss the DNA typing test results of blood samples. Methods The whole blood samples were divided into two groups. The whole blood samples were diluted 5, 10, 20, 25 and 30 times with water to make blood spots. The washing components were pure water hand washing, weak washing soap and strong soap washing 84 Disinfectant dip and washing machine washing 5 kinds of washing methods. All samples were extracted with IQ kit DNA, Identifiler PlusTM kit amplification, and the typing test. Results There was no allele loss in the central part of the hemodilution group. The average peak-height balance was more than 70% except for the 30-fold diluted samples. There were 2 to 10 alleles lost in the peripheral region and peak-height balance Less than 50%. In the washing group, no allele was lost except for the DNA band in the sample of 84 disinfectant washing, while the peak height and the balance were better in hand wash and weak soak samples. Conclusions DNA samples that have been diluted with water or washed with detergent may still be genotyped, even if the pre-benzidine results are negative and the appropriate test site is selected.