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目的 明确非小细胞肺癌 (NSCLC)中相关位点微卫星不稳定性 (MSI)的发生率及其与临床病理指标和预后的相关性。方法 应用PCR -变性聚丙烯酰胺 (PAG)技术检测 94例NSCLC肿瘤组织及相应正常肺组织D3S10 67,D3S65 9,D3S966及AR四个位点的MSI ,并与患者临床病理指标及预后进行对比分析。结果 在所有病例中 64 .89% ( 61/94)呈现至少一个位点的MSI阳性。MSI阳性组与MSI阴性组相比较 ,其淋巴结转移率明显增高 ,差异有显著性意义 ( χ2 =9.945 ,P =0 .0 0 2 )。MSI阳性患者的生存时间显著地低于MSI阴性患者 (P =0 .0 0 0 1)。Cox回归模型分析显示 ,MSI对NSCLC患者是一个重要的预后判断因素 ( χ2 =15 .412 6,P =0 .0 0 0 1) ,淋巴结转移在评估预后中也有一定意义 ( χ2 =6.610 8,P =0 .0 10 1)。结论 MSI与NSCLC患者的淋巴结转移有关 ,并且是其生存率和预后判断的一个重要因素
Objective To determine the incidence of microsatellite instability (MSI) at related sites in non-small cell lung cancer (NSCLC) and its correlation with clinicopathological factors and prognosis. Methods The MSI of D3S10 67, D3S65 9, D3S966 and AR in 94 NSCLC tissues and corresponding normal lung tissues were detected by PCR - polyacrylamide (PAG) technique and compared with the clinicopathological parameters and prognosis . Results In all cases, 64.89% (61/94) showed MSI positive in at least one locus. MSI positive group compared with MSI negative group, the lymph node metastasis rate was significantly increased, the difference was significant (χ2 = 9.945, P = 0.002). The survival time of MSI-positive patients was significantly lower than that of MSI-negative patients (P = 0.0101). Cox regression model analysis showed that MSI was an important prognostic factor for NSCLC patients (χ2 = 15.412 6, P = 0.0101). Lymph node metastasis also had some significance in assessing prognosis (χ2 = 6.610 8, P = 0 .0 10 1). Conclusions MSI is associated with lymph node metastasis in patients with NSCLC and is an important factor in its survival and prognosis
