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目的:建立冰片-葛根素脂质体中葛根素含量及包封率测定方法。方法:采用薄膜分散法制备冰片-葛根素脂质体,葡聚糖凝胶柱层析法分离脂质体和游离药物,采用高效液相色谱法测定脂质体中葛根素的含量和包封率。结果:葛根索在8~160μg·ml~(-1)范围内线性关系良好(r=0.999 8);柱层析法可有效分离药物,分离游离药物的柱回收率为99.32%(RSD=1.16%,n=9),加样回收率为98.56%(RSD=1.23%,n=9)。3批样品的平均包封率为62.35%,65.41%,63.18%(n=3)。结论:该方法准确、灵敏,可用于冰片-葛根素脂质体中葛根素含量及包封率的测定。
Objective: To establish a method for determination of puerarin in puerarin-puerarin liposomes and encapsulation efficiency. Methods: Borneol-puerarin liposomes were prepared by thin-film dispersion method. Liposomes and free drugs were separated by Sephadex LH-20 column chromatography. The content of puerarin in the liposomes was determined by high performance liquid chromatography rate. Results: The calibration curve was linear in the range of 8 ~ 160μg · ml -1 (r = 0.999 8). The column chromatography was effective in the separation of drugs and the column recovery was 99.32% (RSD = 1.16 %, n = 9). The recovery rate was 98.56% (RSD = 1.23%, n = 9). The average entrapment efficiency of three batches of samples was 62.35%, 65.41%, 63.18% (n = 3). Conclusion: This method is accurate and sensitive and can be used for the determination of puerarin content and entrapment efficiency in borneol-puerarin liposomes.