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目的:建立青黛中靛蓝和靛玉红含量的测定方法。方法:采用HPLC定量分析,色谱柱为Diamonsil C18(150mm×4.6mm,5μm),靛蓝流动相为甲醇-水(60∶40),流速为1.0mL·min-1,检测波长为610nm;靛玉红流动相为甲醇-水(70∶30),流速为1.0mL·min-1,检测波长为292nm。结果:靛蓝在0.0296～0.296μg(r=0.9999)与峰面积呈良好线性关系,靛玉红在0.0106～0.1272μg(r=0.9999)与峰面积呈良好线性关系。结论:HPLC操作简便、结果可靠、重现性好、专属性强,可用于青黛中有效成分含量的测定。
Objective: To establish a method for the determination of indigo and indirubin in the indigo. Methods: Quantitative analysis was carried out by HPLC. The chromatographic column was Diamonsil C18 (150mm×4.6mm, 5μm). The indigo-blue mobile phase was methanol-water (60∶40). The flow rate was 1.0mL·min-1. The detection wavelength was 610nm. The red mobile phase was methanol-water (70:30), the flow rate was 1.0 mL·min-1, and the detection wavelength was 292 nm. Results: Indigo showed a good linear relationship with the peak area at 0.0296-0.296 μg (r=0.9999). Indirubin showed a good linear relationship with the peak area at 0.0106-0.1272 μg (r=0.9999). Conclusion: HPLC is simple, reliable, reproducible, and highly specific. It can be used for the determination of active ingredients in the indica.