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目的探讨血管紧张素转换酶抑制剂(ACEI)依那普利是否对 CCl_4引起的大鼠肝纤维化有阻滞作用。方法采用 CCl_4损伤引起的肝硬化门脉高压大鼠模型,随机分为正常对照组、肝硬化模型组、依那普利治疗组。治疗组在用 CCl_4造模的同时用依那普利灌胃,1次/d,直到造模结束,取部分肝组织用甲醛固定后行苏木素-伊红(HE)染色和 Masson 染色并进行肝硬化分级;然后根据染色结果制作组织芯片并行免疫组织化学染色,检测Ⅰ、Ⅲ型胶原的蛋白表达并进行半定量分析。取部分肝组织用逆转录(RT-PCR)法检测Ⅰ、Ⅲ型胶原的 mRNA 表达并进行半定量分析。结果肝硬化分级和 Masson 染色胶原半定量结果证实肝硬化模型组、依那普利治疗组大鼠肝纤维化程度明显加重,与对照组比较差异有统计学意义(P<0.01);而依那普利治疗组的肝纤维化程度较模型组明显减轻,差异有统计学意义(P<0.05)。Ⅰ、Ⅲ型胶原免疫组织化学蛋白表达及 mRNA 表达结果证实肝硬化模型组与对照组、依那普利治疗组比较明显上调(P<0.05)。结论 ACEI 可以有效地下调 CCl_4引起的肝纤维化大鼠的Ⅰ、Ⅲ型胶原的蛋白表达和 mRNA 表达,进而抑制大鼠肝纤维化的发展。
Objective To investigate whether enalapril, an angiotensin converting enzyme inhibitor (ACEI), has a protective effect on hepatic fibrosis induced by CCl_4 in rats. Methods Rat model of cirrhotic portal hypertension induced by CCl 4 injury was randomly divided into normal control group, cirrhosis model group and enalapril treatment group. The treatment group was treated with enalapril at the same time with CCl_4, once / d, until the model was finished. Some of the liver tissues were fixed with formaldehyde, then hematoxylin and eosin (HE) staining and Masson staining were performed and the liver Sclerotic grading; then according to the staining results of tissue chip parallel immunohistochemical staining, detection of type Ⅰ, Ⅲ collagen protein expression and semi-quantitative analysis. A part of liver tissue was taken for detecting the mRNA expression of type I and type III collagen by reverse transcription (RT-PCR) and semi-quantitative analysis. Results The results of liver cirrhosis grading and Masson staining showed that the degree of hepatic fibrosis in cirrhosis model group and enalapril treatment group was significantly higher than that in control group (P <0.01) The degree of hepatic fibrosis in Pulitensis group was significantly lower than that in model group, the difference was statistically significant (P <0.05). Immunohistochemical expression of type I and type III collagen mRNA and mRNA expression results confirmed that liver cirrhosis model group and control group, enalapril treatment group were significantly increased (P <0.05). Conclusion ACEI can effectively down-regulate the protein expression and mRNA expression of type Ⅰ and type Ⅲ collagen in rats with hepatic fibrosis induced by CCl_4, and then inhibit the development of hepatic fibrosis in rats.