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目的探讨丙二醛(MDA)、总抗氧化能力(T-AOC)等氧化损伤生化指标筛检煤工尘肺的价值,为早期发现可疑患者和煤工尘肺的防治提供可能的线索。方法分别采用硝酸还原酶法、硫代巴比妥酸显色法和化学比色法测定112例煤工尘肺患者和144例健康接尘对照血浆氧化损伤指标MDA、T-AOC及诱导型一氧化氮合酶(iNOS)的含量或活力。采用判别分析法判定3项指标的联合筛检价值。结果 3项氧化损伤指标MDA、T-AOC和iNOS联合构建的判别方程,筛检煤工尘肺的特异度为89.1%,灵敏度为77.7%,符合率为84.0%,均高于单一指标和T-AOC与MDA的串联,差异有统计学意义(P<0.05)。结论 MDA、T-AOC和iNOS联合筛检煤工尘肺价值均高于单一指标和T-AOC与MDA的串联。
Objective To investigate the value of biochemical indicators of oxidative damage such as malondialdehyde (MDA) and total antioxidant capacity (T-AOC) in the screening of coal workers ’pneumoconiosis, and to provide possible clues for the early detection of suspected patients and coal workers’ pneumoconiosis. Methods Nitric acid reductase, thiobarbituric acid colorimetric method and chemical colorimetry were used to determine the plasma levels of MDA, T-AOC and inducible monooxygenase in 112 coal workers with pneumoconiosis and 144 healthy controls. Nitrogen synthase (iNOS) content or viability. Discriminant analysis was used to determine the joint screening value of 3 indicators. Results Three discriminant equations of MDA, T-AOC and iNOS were constructed. The specificity of coal worker’s pneumoconiosis was 89.1%, the sensitivity was 77.7% and the coincidence rate was 84.0%, which were all higher than those of single indicator and T- AOC and MDA in series, the difference was statistically significant (P <0.05). Conclusion The combined detection of MDA, T-AOC and iNOS in coal miners’ pneumoconiosis was higher than that of single indicator and tandem T-AOC and MDA.