论文部分内容阅读
目的：探讨碱性成纤维细胞生长因子（ｂＦＧＦ）对庆大霉素（ＧＭ）肾毒性的保护作用。方法：豚鼠２０只，随机分为ＧＭ组和ＧＭ＋ｂＦＧＦ组各１０只，ＧＭ组按每只８０ｍｇ／ｋｇｄ－１肌注ＧＭ１７ｄ，停药后继续注射生理盐水６ｄ；ＧＭ＋ｂＦＧＦ组在肌注ＧＭ的同时加肌注２０００Ｕ／ｋｇｄ－１ｂＦＧＦ１７ｄ，停ＧＭ后继续注射ｂＦＧＦ６ｄ。用药前、后称体质量，并做统计学ｔ检验：断头处死动物后取肾皮质做光镜和电镜观察。结果：ＧＭ组动物出现急性肾功能衰竭，用药前、后体质量变化有显著性意义（ｔ＝４．３４６３，Ｐ＜０．０５），形态上肾小管上皮细胞明显损害；ＧＭ＋ｂＦＧＦ组动物无急性肾功能衰竭症状，用药前、后体质量变化无显著性意义（ｔ＝０．３８９３，Ｐ＞０．０５），形态上肾小管上皮细胞基本正常。结论：碱性成纤维细胞生长因子对庆大霉素肾毒性有保护作用。
Objective: To investigate the protective effect of basic fibroblast growth factor (bFGF) on gentamicin (GM) nephrotoxicity. Methods: Twenty guinea pigs were randomly divided into GM group and GM + bFGF group, 10 rats in each group. GM group was injected intramuscularly with GM 80 mg / kgd-1 for 17 days, and then injected with normal saline for 6 days. GM + bFGF group Intramuscular injection of 2000U / kgd-1bFGF17d, stop GM injection of bFGF6d. Before and after treatment, weighed the body weight, and did statistical t-test: The animals were sacrificed and the kidneys were sacrificed after decapitation. Results: The rats in GM group had acute renal failure, and the changes of body weight before and after treatment were significant (t = 4.3463, P <0.05). The morphological renal tubular epithelial cells were obviously damaged. The animals in GM + bFGF group had no acute Renal failure, medication before and after the change in body weight was not significant (t = 0.3893, P> 0.05), the morphology of renal tubular epithelial cells were normal. Conclusion: Basic fibroblast growth factor has a protective effect on gentamicin nephrotoxicity.