论文部分内容阅读
棉花黄萎病是一种极难防治的土传性真菌病害,研究病原菌侵染棉花的组织学过程对致病机理解析和抗病资源利用具有重要意义。本研究利用绿色荧光蛋白标记的大丽轮枝菌系统研究了其对抗病棉种海岛棉7124和三裂棉、感病棉种军棉1号和戴维逊棉的侵染过程。结果表明,大丽轮枝菌对抗/感棉种的初始侵染没有明显差异,接菌5h后,分生孢子均能吸附在感病和抗病棉种的根表面。但侵染过程存在显著差异,侵染感病棉种中病原菌3~5 d到达皮层,5~7 d达到维管束,随后迅速扩展繁殖,侵染14 d后即完成系统侵染,并开始产生黄萎病症状;而病原菌侵染抗病棉种,5~7d才侵入皮层,7~10 d到达维管束,14 d后仍无法扩展,病原菌的定殖与发展受到限制,无法形成系统侵染,较少形成黄萎病症状。本研究通过绿色荧光蛋白标记大丽轮枝菌对抗/感棉种的侵染过程研究,为大丽轮枝菌致病机理研究和抗性资源利用提供了强有力的理论依据。
Verticillium dahliae is an extremely difficult to control soil-transmitted fungal disease. Studying the histological process of pathogen-infected cotton is of great significance to the analysis of pathogenic mechanism and utilization of disease-resistant resources. In this study, the green fluorescent protein-labeled strains of Verticillium dahliae were used to study the infection process of diseased cotton varieties of island cotton 7124 and tripartite, susceptible cotton Junmian 1 and Davidson. The results showed that there was no significant difference in initial infection between resistant and susceptible cottons to Verticillium dahliae. After 5 hours of inoculation, conidia could be adsorbed on the root surface of susceptible and resistant cotton species. However, there was a significant difference in the infection process. Pathogenic bacteria in susceptible cotton species reached the cortex 3-5 days after reaching the cortex and reached vascular bundles 5 to 7 days later, and then rapidly expanded to proliferate. After 14 days of infection, the system was infected and started to produce Verticillium wilt disease. Pathogens invaded the resistant cotton species and invaded the cortex only 5 ~ 7 days later, reached the vascular bundle 7 ~ 10 days later and still could not be expanded after 14 days. The colonization and development of pathogenic bacteria were restricted and could not form systemic infection , Fewer symptoms of verticillium wilt formation. In this study, the green fluorescent protein-labeled Verticillium dahliae resistant / susceptible varieties of cotton infection process for the pathogenic mechanism of Verticillium dahliae research and utilization of resistant resources provides a powerful theoretical basis.