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目的构建并扩增pcDNA3.1-FKBP12.6基因表达载体。方法目的基因全基因合成,PCR加入酶切位点,连接入pGEM-Teasy载体,经酶切鉴定及测序后获得测序正确的目的基因。连接入真核表达载体pcDNA3.1(-),再经酶切鉴定及测序后进行pcDNA3.1-FKBP12.6的扩增。结果成功构建并扩增pcDNA3.1-FKBP12.6基因表达载体。结论pCDNA3.1-FK-BP12.6表达载体的构建,为深入研究FKBP12.6基因对心肌细胞结构和功能的影响奠定基础,进而为探索安全、高效的心肌功能异常的治疗提供新的途径。
Objective To construct and amplify pcDNA3.1-FKBP12.6 gene expression vector. Methods The gene of interest was synthesized by PCR and inserted into the restriction enzyme sites of pGEM-Teasy. The target gene was sequenced and sequenced. Connected to the eukaryotic expression vector pcDNA3.1 (-), and then identified by the restriction enzyme digestion and sequencing of pcDNA3.1-FKBP12.6 amplification. Results The pcDNA3.1-FKBP12.6 gene expression vector was successfully constructed and amplified. Conclusion The construction of pCDNA3.1-FK-BP12.6 expression vector lays the foundation for further study of the effect of FKBP12.6 gene on the structure and function of cardiomyocytes, and thus provides a new way to explore the safe and efficient treatment of myocardial dysfunction.