论文部分内容阅读
目的:探讨整合素连接激酶(ILK)修饰的骨髓间充质干细胞(MSCs)对心肌梗死后心脏心功能的影响。方法:取大鼠骨髓体外分离扩增培养MSCs,分别用ILK、ILK-siRNA及对照GFP腺病毒转染MSCs。结扎大鼠心脏左前降支1h后,分别将GFP-MSCs、ILK-MSCs、ILK-siRNA-MSCs多点注射到大鼠心肌梗死区周边,对照组注射等量PBS。结果:细胞移植4d后与GFP-MSCs移植组相比,ILK-MSCs组存活细胞数目明显增多,而ILKsiRNA-MSCs移植组存活细胞明显减少(P<0.05)。细胞移植3周后与GFP-MSCs移植组相比,ILK-MSCs组新生血管数目明显增多,且胶原蛋白沉积减少,梗死面积明显缩小,心功能各项指标明显改善(P<0.05);而ILK-siRNA-MSCs移植组新生血管数目明显减少,胶原蛋白沉积增多,梗死面积明显增加,心功能各项指标明显恶化(P<0.05)。结论:ILK增加MSCs存活率、促进梗死后心脏血管新生,从而抑制心室重构,改善心功能。
Objective: To investigate the effects of integrin-linked kinase (ILK) modified bone marrow mesenchymal stem cells (MSCs) on cardiac function after myocardial infarction. Methods: MSCs were isolated from rat bone marrow and cultured in vitro. MSCs were transfected with ILK, ILK-siRNA and control GFP adenovirus respectively. After ligation of left anterior descending branch of rat heart for 1h, GFP-MSCs, ILK-MSCs and ILK-siRNA-MSCs were injected into the periphery of myocardial infarction area and the control group were injected with equal volume of PBS. Results: Compared with GFP-MSCs transplantation group, the number of viable cells in ILK-MSCs group increased significantly 4 days after cell transplantation, while the number of surviving cells in ILKsiRNA-MSCs transplantation group decreased significantly (P <0.05). Compared with GFP-MSCs transplantation group, the number of neovascularization in ILK-MSCs group was significantly increased after 3 weeks of cell transplantation, the collagen deposition was decreased, the infarct size was significantly reduced, and the indexes of cardiac function were significantly improved (P <0.05) The number of neovascularization in the siRNA-MSCs transplantation group was significantly reduced, collagen deposition increased, infarct size increased significantly, and cardiac function indicators were significantly worsened (P <0.05). Conclusion: ILK increases the survival rate of MSCs and promotes angiogenesis after infarction, thus inhibiting ventricular remodeling and improving cardiac function.