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目的探讨蛛网膜下腔出血后氧化应激链中兴奋性氨基酸对脑微循环的影响。方法颈内血管穿刺法制作大鼠蛛网膜下腔出血模型。实验共分3组,出血模型3天组与急性期组(对照)。其中,出血后3天组又分2组,一组大鼠确定海马CA3区,另一组确定侧脑室区,分别采用微量进样器直接注射兴奋性氨基酸类似物海人酸(KA)与肾上腺髓质素(ADM),激光多普勒监测,观察并对比三者脑表面局部血流灌注量的变化趋势。结果ADM能提高SAH后脑表面局部微循环血流灌注量,KA能、降低其脑表面局部微循环血流灌注量。结论SAH后产生的氧化应激链中兴奋性氨基酸能够显著降低脑表面局部微循环血流灌注量。
Objective To investigate the effect of excitatory amino acids in the oxidative stress chain on cerebral microcirculation after subarachnoid hemorrhage. Methods The rat models of subarachnoid hemorrhage were made by intracranial vascular puncture. The experiment was divided into 3 groups, 3-day bleeding model group and acute phase group (control). Among them, 3 days after hemorrhage group was divided into 2 groups, one group of rats to determine the hippocampal CA3 area, the other group to determine the lateral ventricle area, respectively, using a microinjection injector direct injection of excitatory amino acid analogs kainate (KA) and adrenal (ADM), laser Doppler monitoring, observation and comparison of the three local cerebral blood flow perfusion changes. Results ADM increased the local microcirculation flux and KA energy on the surface of the brain after SAH, and decreased the local microcirculation perfusion on the brain surface. Conclusions Excitatory amino acids in the oxidative stress chain produced after SAH can significantly reduce the local microcirculation perfusion.