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目的建立移植大鼠生精干细胞到裸鼠生精小管的实验系统。方法采用一次腹腔注射busulfan 30mg/kg以消除裸鼠生精小管内源性生精细胞,制备生精干细胞移植受体小鼠;采用laminin粘附的方法分离大鼠生精干细胞;应用生精小管直接注射和输出管注射的方法移植分离到的大鼠生精干细胞进入受体裸鼠的生精小管内。结果busulfan腹腔注射4周后受体裸鼠生精小管中的精子发生明显得到抑制。大鼠生精干细胞移植2～3个月后在受体裸鼠的生精小管中发现了大鼠的长型精子细胞。结论成功建立了移植大鼠生精干细胞到受体裸鼠生精小管的试验系统。
Objective To establish an experimental system of transplanted rat spermatogenic stem cells into the seminiferous tubules of nude mice. Methods A single intraperitoneal injection of busulfan 30 mg / kg was used to eliminate endogenous spermatogenic cells of the seminiferous tubules of nude mice, and the stem cells were transplanted into recipient mice. The spermary stem cells were isolated by laminin adhesion method. Methods of direct injection and delivery tube injection The isolated rat spermatogenic stem cells were transplanted into the seminiferous tubules of the recipient nude mice. Results Busulfan spermatogenesis was significantly inhibited in the seminiferous tubules of nude mice 4 weeks after intraperitoneal injection of busulfan. Rat spermatogonial stem cells 2 to 3 months after transplantation in the recipient seminiferous tubules of rats found in the long sperm cells. Conclusion The experimental system of transplanted spermatogenic stem cells into the seminiferous tubules of nude mice was successfully established.