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目的:以胎牛血清(Fetal bovine serum,FBS)涂层为标准对照,研究minTBP-1-PRGDN双靶向融合肽对成骨细胞粘附的影响。方法:对商用纯钛进行minTBP-1-PRGDN双靶向融合多肽涂层,以无涂层的钛表面及胎牛血清涂层的钛表面分别作为空白对照和阳性对照,比较不同涂层处理对成骨细胞附着及伸展的影响。结果:成骨细胞粘附1h后,minTBP-1-PRGDN融合肽涂层的钛片上的细胞附着数量最多,FBS涂层表面次之,无涂层的钛片表面细胞数量最少、且与minTBP-1-PRGDN组比较有统计学差异(P<0.05);经形态计量学分析:FBS组细胞伸展面积最大,minTBP-1-PRGDN组次之,无涂层组细胞伸展最小。结论:minTBP-1-PRGDN融合肽涂层能提高成骨细胞在钛表面的附着和伸展能力。
OBJECTIVE: To investigate the effect of minTBP-1-PRGDN double-targeting fusion peptide on osteoblast adhesion by Fetal bovine serum (FBS) coating as a standard control. Methods: The minTBP-1-PRGDN double-targeting fusion polypeptide was coated on commercial pure titanium. The uncoated titanium surface and the fetal bovine serum-coated titanium surface were used as blank control and positive control respectively. Effect of osteoblast attachment and extension. RESULTS: After 1 h of osteoblast attachment, the number of cells attached to the minTBP-1-PRGDN fusion peptide coating was the highest on the titanium surface. The number of cells on the surface of the FBS coating was the second, 1-PRGDN group (P <0.05). Morphometry analysis showed that the cell extension area in FBS group was the largest, followed by minTBP-1-PRGDN group, and the cell spreading in the group without coating was the smallest. Conclusion: minTBP-1-PRGDN fusion peptide coating can enhance the osteoblasts adhesion and extension on titanium surface.