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AIM:To evaluate the therapeutic effect of adenoviral-vector-delivered human interleukin-10(hIL-10)gene on severeacute pancreatitis(SAP)rats.METHODS:Healthy Sprague-Dawley(SD)rats wereintraperitoneally injected with adenoviral IL-10 gene(AdvhIL-10),empty vector(Adv0)or PBS solution.Blood,liver,pancreas and lung were harvested on the secondday to examine hIL-10 level by ELISA and serum amylaseby enzymatic assay.A SAP model was induced by retrogradeinjection of sodium taurocholate through pancreatic duct.SAP rats were then administered with AdvhIL-10,Adv0and PBS solution by a single intraperitoneal injection 20 minafter SAP induction.In addition to serum amylase assay,levels of hIL-10 and tumor necrosis factor-α(TNF-α)weredetected by RT-PCR,ELISA and histological study.Themortality rate was studied and analyzed by Kaplan-Meierand log rank analysis.RESULTS:The levels of hIL-10 in the pancreas,liver andlung of healthy rats increased significantly after AdvhIL-10injection(1.42 ng/g in liver,0.91 ng/g in pancreas);whilethere was no significant change of hIL-10 in the other twocontrol groups.The concentration of hIL-10 was increasedsignificantly in the SAP rats after AdvhIL-10 injection(1.68 ng/g in liver,1.12 ng/g in pancreas)compared tothe other two SAP groups with blank vector or PBStreatment(P<0.05).The serum amylase levels remainednormal in the AdvhIL-10 transfected healthy rats.However,the serum amylase level was significantly elevated in theother two control SAP rats.In contrast,serum amylasewas down-regulated in the AdvhIL-10 treated SAP groups.The TNF-α expression in the AdvhIL-10 treated SAP ratswas significantly lower compared to the other two controlSAP groups.The pathohistological changes in the AdvhIL-10 treated group were better than those in the other twocontrol groups.Furthermore,the mortality of the AdvhIL-10 treated group was significantly reduced compared tothe other two control groups(P<0.05).CONCLUSION:Adenoviral hIL-10 gene can significantlyattenuate the severity of SAP rats,and can be used in the treatment of acute inflammation process.
AIM: To evaluate the therapeutic effect of adenoviral-vector-delivered human interleukin-10 (hIL-10) gene on severe acute pancreatitis (SAP) rats. METHODS: Healthy Sprague-Dawley AdvhIL-10), empty vector (Adv0) or PBS solution. Blood, liver, pancreas and lung were harvested on the secondday to examine hIL-10 level by ELISA and serum amylaseby enzymatic assay. A SAP model was induced by retrograde injection of sodium taurocholate through pancreatic duct. SAP rats were then administered with AdvhIL-10, Adv0 and PBS solution by a single intraperitoneal injection 20 mL of SAP induction. addition to serum amylase assay, levels of hIL-10 and tumor necrosis factor-α (TNF- weredetected by RT-PCR, ELISA and histological study. The rate of washes and analyzed by Kaplan-Meierand log rank analysis .RESULTS: The levels of hIL-10 in the pancreas, liver and lung of healthy rats increased significantly after AdvhIL-10 injection ng / g in live 0.91 ng / g in pancreas); while there was no significant change of hIL-10 in the other twocontrol groups. The concentration of hIL-10 was increased significantly in the SAP rats after AdvhIL-10 injection (1.68 ng / g in liver, 1.12 ng / g in pancreas) compared tothe other two SAP groups with blank vector or PBStreatment (P <0.05). The serum amylase levels remained normal in the AdvhIL-10 transfected healthy rats. Despite the serum amylase level was significantly elevated in the other two control SAP rats. In contrast, serum amylase was down-regulated in the AdvhIL-10 treated SAP groups. The TNF-α expression in the AdvhIL-10 treated SAP rats was significantly lower compared to the other two control SAP groups. The pathohistological changes in the AdvhIL -10 treated group were better than those in the other twocontrol groups. Still more, the mortality of the AdvhIL-10 treated group was significantly reduced compared to the other two control groups (P <0.05) .CONCLUSION: Adenoviral hIL-10 gene can significantlyattenua tethe severity of SAP rats, and can be used in the treatment of acute inflammation process.