论文部分内容阅读
红麻是最重要的自然纤维作物之一,然而SSR标记的匮乏限制了其遗传改良。本研究从红麻90 175个EST序列中挑出含有转录因子的EST,开发了94对SSR引物。以24份不同红麻种质资源的DNA为模板,利用9%非变性聚丙烯酰胺凝胶电泳检测多态性。结果表明,85对引物(占90.4%)至少在2个材料之间存在多态性,表明开发的EST-SSR具有很好的多态性。其中,三核苷酸重复所占比例最多,重复基元AAT和ATG的多态性较高。聚类分析表明,24份红麻种质资源的遗传相似系数变化在0.62~0.92之间,表现出丰富的遗传基础。这些结果不仅丰富了红麻的分子标记数量,而且为红麻的遗传分析提供资源。
Kenaf is one of the most important natural fiber crops, however, the lack of SSR markers limits its genetic improvement. In this study, ESTs containing transcription factors were selected from 90 175 EST sequences of kenaf and 94 pairs of SSR primers were developed. Twenty-four DNAs of different kenaf germplasm resources were used as templates to detect polymorphisms using 9% non-denaturing polyacrylamide gel electrophoresis. The results showed that 85 pairs of primers (90.4%) had polymorphism between at least two materials, indicating that EST-SSR developed had good polymorphism. Among them, trinucleotide repeat accounted for the largest proportion of repeat motifs AAT and ATG higher polymorphism. Cluster analysis showed that the genetic similarity coefficient of 24 kenaf germplasm resources varied from 0.62 to 0.92, showing a rich genetic basis. These results not only enrich the number of kenaf molecular markers, but also provide resources for the genetic analysis of kenaf.