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目的研究Babesia Microti和Babesia Rodhaini感染的交叉免疫保护作用,以探讨B.Microti和B.Rodhaini不同种属之间是否存在交叉保护及保护机制。方法首先制备Babesia Microti感染耐过鼠,再用Babesia Rodhaini进行再感染,分别于B.Rodhaini再感染后的12、24、48、72h,使用ELISA方法分别检测血清中IL-12及IL-10的浓度,并同时检测寄生虫血症、网织红细胞变化、致死率与死亡时间。结果B.Microti感染耐过鼠再感染Babesia Rodhain后24h,小鼠血液中IL-12较未感染组及B.Microti感染耐过鼠明显增高,而血液中IL-10水平在再感染后72h时与对照组比较差异显著。B.Microti感染耐过鼠再感染Babesia Rodhain后,血液中出现一过性寄生虫血症,实验鼠可完全耐过。结论B.Microti感染耐过鼠可完全保护B.Rodhain的再感染,其细胞因子的产生为IL-12早于IL-10。
Objective To study the cross immunoprotective effect of Babesia Microti and Babesia Rodhaini infection in order to find out whether cross protection and protection mechanisms exist between different species of B. micrroti and B. Rodhaini. Methods Babesia Microti infection-resistant mice were prepared and then re-infected with Babesia Rodhaini. Serum IL-12 and IL-10 levels were detected by ELISA at 12, 24, 48 and 72 hours after re-infection with B. Rodhaini. Concentration, and at the same time detect parasitemia, reticulocyte changes, lethality and death time. Results B. 24h after mice were infected with Microti, the level of IL-10 in blood was significantly higher than that in non-infected mice and B.Microti infected mice, but the levels of IL-10 in blood at 72h after re-infection Compared with the control group, the difference was significant. B. Microti infection resistant mice and then infected with Babesia Rodhain, the blood appeared transient parasitosis, mice can be completely resistant. B. Microti infected mice can completely protect B.Rodhain re-infection, the production of cytokines as IL-12 earlier than IL-10.