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目的探讨let-7靶序列KRAS 3′非翻译区rs712多态性与宫颈癌发病的相关性。方法收集204例宫颈癌患者和218例健康对照人群外周静脉血,提取基因组DNA,采用聚合酶链反应-限制性片段长度多态性方法对KRAS rs712进行分型。结果 TT和GT/TT基因型在宫颈癌组中的频率分别为9.3%和45.1%,显著高于其在对照组中的频率,分别为2.8%和33.5%(TT与GG相比:χ2=9.63,P=0.002;GT/TT与GG相比:χ2=5.97,P=0.015)。等位基因T在宫颈癌组中的频率为27.2%,显著高于其在对照组中的频率18.1%(T与G相比:χ2=9.98,P=0.002)。结论 KRAS rs712多态性与宫颈癌的发病有关。
Objective To investigate the relationship between rs712 polymorphism of KRAS 3 ’untranslated region and the incidence of cervical cancer in let-7 target sequence. Methods Peripheral venous blood was collected from 204 patients with cervical cancer and 218 healthy controls. Genomic DNA was extracted and KRAS rs712 was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The frequencies of TT and GT / TT genotypes in cervical cancer group were 9.3% and 45.1%, respectively, which were significantly higher than those in control group (2.8% and 33.5% respectively) (TT vs GG: χ2 = 9.63, P = 0.002; GT / TT vs. GG: χ2 = 5.97, P = 0.015). The frequency of allele T in cervical cancer was 27.2%, significantly higher than its frequency in controls of 18.1% (T vs G: χ2 = 9.98, P = 0.002). Conclusion KRAS rs712 polymorphism is associated with the development of cervical cancer.