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目的建立抗生素敏感性微生物源追踪方法,以分析淮河流域某水库粪便污染来源。方法采集水库周边已知来源粪便标本,分离指示菌,接种至含抗生素的培养基,筛选能区分不同来源指示菌的抗生素浓度,用JMP7.0软件进行判别分析检验效果。同时采集水样进行粪便污染来源追踪。结果筛选的不同抗生素及其浓度能将已知来源指示菌正确归类率分别为93.05%(2分类)、85.75%(3分类)和78.53%(9分类)。100株指示菌中有58株来自家畜,25株来自家禽,11株来自狗,4株来自人,2株来自野生动物。水样标本中主要的粪便污染来源为猪、牛、鸡,分别占水样分离指示菌总数的36%,17%和15%。结论所筛选的抗生素浓度均能较好地区分指示菌的不同来源,该方法的建立可以为查找水体粪便污染来源以及水体治理提供参考与帮助。
Objective To establish an antibiotic-sensitive microbial source tracking method to analyze the source of fecal contamination in a reservoir in the Huaihe River Basin. Methods Stool samples from known sources around the reservoir were collected, and the indicator bacteria were isolated and inoculated into the medium containing antibiotics. The antibiotic concentration of the indicator bacteria from different sources was screened by screening, and the discriminant analysis results were verified by JMP7.0 software. At the same time collecting water samples for stool pollution source tracking. Results The correct antibiotics and their concentrations were screened by 93.05% (two categories), 85.75% (three categories) and 78.53% (nine categories) respectively. Of the 100 strains of indicator bacteria, 58 were from domestic animals, 25 were from poultry, 11 were from dogs, 4 were from humans and 2 were from wild animals. The main source of faecal contamination in water samples is pigs, cattle and chickens, accounting for 36%, 17% and 15% of the total number of water-soluble indicator bacteria. Conclusion The screening of antibiotic concentrations can distinguish different sources of indicator bacteria well. The establishment of this method can provide reference and help for finding sources of water pollution and water treatment.