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介体灰飞虱的亚显微结构及水稻条叶枯病毒(RSV)病害特异性蛋白SP的免疫定位显示:带毒虫卵巢内的成熟卵含大量内共生菌,证实了它的经卵传递特征.同时在卵的外周、卵内以及中肠的肠腔和肠上皮细胞内均有胶体金颗粒分布.说明RSV在介体内的循回途径和经卵传递的特征.对照组的雌虫则仅含内共生菌,而无胶体金颗粒分布;雄虫则不论带毒与否,其精子均不含内共生菌,也无胶体金颗粒标记.这为灰飞虱经卵传毒给后代的生物测试提供了直观的实验证据,也为传毒机理研究和探讨RSV蛋白的功能奠定了必要的科学基础.
The sub-microstructure of the Laodelphax striatellus and the immunolocalization of the disease-specific protein SP of the rice leaf blight virus (RSV) showed that mature eggs in the ovary of the poisonous larvae contained a large amount of endosymbiotic bacteria, confirming their ovo transfer characteristics . At the same time, there are colloidal gold particles distributed in the periphery of the egg, the egg and the intestinal lumen and intestinal epithelial cells in the midgut. Explain the characteristics of RSV’s pathways and ovum transference within the mediator. The control group of females contained only endosymbiontic, but no colloidal gold particles; male or infected with or without sperm are not containing endosymbionts, no colloidal gold particles labeled. This provided intuitionistic experimental evidence for the bioassay of Laodelphax striatellus to the offspring and lays the necessary scientific foundation for the study of the mechanism of virus transmission and the function of RSV protein.