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本实验采用继代多年的花培体细胞无性系Hu18再生绿芽(0.5mm)为起始材料,研究多效唑(MET)与其它激素配合使用对试管苗的调控作用,结果指出:(1)单独使用MET对绿芽生长有毒害作用,除2,4-D、GA,外,MET与适宜浓度的其它激素配合使用才能发挥增苗、壮苗作用,其中以MET与BA配合使用的培养效果最好,MET与NAA,C_2H_4配合使用的效果次之;(2)MET与其他激素配合使用不但能降低植株高度,促进根系发育,而且可以延长试管苗的保存时间;(3)MET与乙烯利配合使用能加速绿芽成苗速度,而与其他激素配合使则延缓绿芽成苗速度,如与2,4-D配合使用则延缓2,4-D对绿芽的脱分化进程;(4)在本实验条件下,以MET 2.5mg/L+BA 2mg/L+NAA 0.2mg/L配合使用有利根芽的协调生长。本文还从植株干物质累积,叶细胞结构,细胞活力等方面进行了探讨。
In this experiment, Hu18 regenerated green shoot (0.5mm), which has been used for many years, was used as a starting material to study the regulatory effect of MET and other hormones on plantlets. The results showed that: (1) The use of MET toxic effects on the growth of green shoots, in addition to 2,4-D, GA, MET, and appropriate concentrations of other hormones in order to play to increase seedlings, strong seedlings role, with MET and BA with the most effective culture (2) The combination of MET and other hormones can not only reduce plant height and promote root development, but also prolong the preservation time of tube seedlings; (3) The combination of MET and ethephon When used with 2,4-D, the rate of dedifferentiation of green shoots was slowed down; (4) Under the experimental conditions, coordinated growth of favorable root buds with MET 2.5 mg / L + BA 2 mg / L + NAA 0.2 mg / L was used. This article also from the plant dry matter accumulation, leaf cell structure, cell viability and other aspects were discussed.