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目的:采用实验方法观察推拿对应激大鼠亚健康样行为和HPA轴相关激素水平的影响。方法:将60只wistar大鼠随机分为正常组、模型组和推拿组,后两组予束缚应激,推拿组予背部循经推拿。实验前1 d及第7、14、21天利用鼠尾悬挂试验,实验前1 d及第21天利用旷场试验,分别观察3组大鼠的行为学改变。实验结束后放免法测血清促肾上腺皮质激素(ACTH)、皮质酮(CORT)水平。结果:旷场试验结果:实验结束后,模型组大鼠中央格停留时间明显增加,跨格次数、修饰次数及修饰时间减少,与实验前组内比较,差异有统计学意义(P<0.05);与正常组比较,差异有统计学意义(P<0.05)。与实验后模型组比较,推拿组的中央格停留时间减少,跨格次数、修饰次数及修饰时间增加,差异有统计学意义(P<0.05)。鼠尾悬挂试验结果:实验结束后模型组大鼠不动时间明显增加,挣扎次数明显减少,与实验前比较,差异有统计学意义(P<0.05);实验结束后模型组挣扎次数明显少于正常组(P<0.05),不动时间明显多于正常组(P<0.05);随着造模时间的延长,大鼠悬尾试验不动时间增加、挣扎次数减少,而推拿组不动时间则显著短于同期模型组(P<0.05),挣扎次数则多于同期模型组(P<0.05)。3组大鼠实验结束后血清CORT、ACTH水平检测结果:模型组大鼠血清CORT浓度明显高于正常组,差异有统计学意义(P<0.05);推拿组大鼠血清CORT浓度明显低于模型组,差异有统计学意义(P<0.05)。模型组、推拿组血清ACTH浓度明显高于正常组,差异均有统计学意义(P<0.05);推拿组血清ACTH浓度明显低于模型组,差异有统计学意义(P<0.05)。结论:背部循经推拿手法能改善应激模型大鼠的躯体与精神疲劳状态,可能作用机制为调节HPA轴异常的功能活动。
OBJECTIVE: To observe the effects of massage on the sub-health-like behaviors and the levels of HPA-related hormones in stress-induced rats using experimental methods. Methods: Sixty wistar rats were randomly divided into normal group, model group and massage group. The latter two groups were given restraint stress, and the massage group was given back massage. The rat tail suspension test was performed on the 1st day and the 7th, 14th and 21st day before the experiment and the open field test on the 1st day and the 21st day before the experiment to observe the behavioral changes of the three groups respectively. At the end of the experiment, serum adrenocorticotropic hormone (ACTH) and corticosterone (CORT) levels were measured by radioimmunoassay. Results: The result of open-field test: After the experiment, the retention time of the central grid in the model group increased significantly, the number of crossover times, the number of modification and the modification time decreased. Compared with the pre-test group, the difference was statistically significant (P <0.05) ; Compared with the normal group, the difference was statistically significant (P <0.05). Compared with the model group, the retention time of the central cell of the massage group decreased, the number of times of interclassification, the times of modification and the time of modification increased, the difference was statistically significant (P <0.05). The rat tail suspension test results: After the experiment, the rats in the untreated group increased their immobility time and the number of struggles significantly reduced, compared with those before the experiment (P <0.05); the numbers of struggling in the model group after the experiment were significantly less than those in the model group Normal group (P <0.05), immobility time was significantly more than the normal group (P <0.05); with modeling time extension, rat tail suspension test increased immobility time, the number of struggles decreased, while the massage group does not move time Was significantly shorter than the model group (P <0.05), the number of struggles was more than the model group (P <0.05). The levels of serum CORT and ACTH in the three groups of rats at the end of the experiment showed that the concentration of serum CORT in the model group was significantly higher than that in the normal group (P <0.05), and the level of serum CORT in the model group was significantly lower than that of the model Group, the difference was statistically significant (P <0.05). The levels of serum ACTH in model group and massage group were significantly higher than those in normal group (P <0.05). The serum ACTH concentration in massage group was significantly lower than that in model group (P <0.05). CONCLUSION: Back massage can improve the physical and mental fatigue of stress rats. The possible mechanism is the functional activity that regulates the abnormality of HPA axis.