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目的研究中链脂肪酸(MCFA)中辛酸或癸酸(C10:0)对体质量的影响及机制。方法高脂肪饲料喂养C57BL/6J小鼠,建立肥胖小鼠模型,随机分为3组,在高脂饲料基础上分别添加2%辛酸(C8:0),2%癸酸(C10:0),或2%油酸(C18:1)。每周监测两次体质量和饲料消耗量。8 w后,测体质量、体脂肪重及与脂解作用相关基因在白色脂肪组织(WAT)中蛋白和m RNA表达。结果癸酸(C10:0)组体重,Lee’s指数,BMI和附睾周围脂肪组织重量显著低于油酸(C18:1)组,WAT中脂肪甘油三酯脂肪酶(ATGL),激素敏感脂肪酶(HSL),环磷酸腺苷(c AMP)和β3肾上腺素能受体(β3-AR)水平显著高于油酸(C18:1)组。辛酸(C8:0)与油酸(C18:1)组比较,均无显著性差异。结论 MCFA的癸酸(C10:0),而不是辛酸(C8:0),可以有效地减少肥胖小鼠体质量,机制可能是上调了WAT中的β3-AR,ATGL和HSL表达,促进了脂解作用。
Objective To study the effect of octanoic acid or capric acid (C10: 0) on the body mass of medium chain fatty acids (MCFA) and its mechanism. Methods C57BL / 6J mice were fed with high fat diet. The model of obese mice was established and divided into 3 groups randomly. On the basis of high fat diet, 2% octanoic acid (C8: 0), 2% Or 2% oleic acid (C18: 1). Body weight and feed consumption are monitored twice weekly. After 8 weeks, the body mass, body fat mass, and the expression of protein and m RNA in the white adipose tissue (WAT) related to lipolysis were measured. Results Body weight, Lee’s index, BMI and peri-epididymal adipose tissue weight in the capric acid (C10: 0) group were significantly lower than those in the oleic acid (C18: 1) group, WAT triglyceride lipase (ATGL), hormone sensitive lipase HSL, c AMP and β3-adrenoceptor (β3-AR) were significantly higher than those in oleic acid (C18: 1) group. There was no significant difference between octanoic acid (C8: 0) and oleic acid (C18: 1) group. Conclusion C10: 0, but not octanoic acid (C8: 0), can effectively reduce the body weight of obese mice. The mechanism may be that the up-regulation of β3-AR, ATGL and HSL expression in WAT promoted the lipid Solution.