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为了明确灰霉病菌对新型杀菌剂啶菌恶唑的敏感性和该药剂与其它杀菌剂的交互抗性,采用菌丝生长速率法测定河北省不同地区的95个灰霉菌菌株对啶菌恶唑的敏感性,并测定11个相对敏感菌株和3个相对抗性菌株对啶菌恶唑、嘧霉胺、速克灵、多菌灵和苯醚甲环唑的敏感性。结果表明,灰霉菌菌株间对啶菌恶唑的敏感性差异显著,EC50值在0.0693～3.0578μg/mL之间,平均为0.2045μg/mL,其中3个灰霉菌株EC50值分别为平均EC50值的7.97、13.06和14.95倍;啶菌恶唑与上述不同作用机制杀菌剂之间的相关系数很低,不存在交互抗性。在无药PDA培养基上继代培养11代后,3个相对抗性菌株的敏感性明显提高,表明其抗药性不能稳定遗传。
In order to clarify the sensitivity of Botrytis cinerea to the new fungicide dacoxazole and its interaction with other fungicides, 95 mycotoxins strains from different areas of Hebei Province were tested for mycobactoxazole The sensitivity of 11 relative sensitive strains and 3 relative resistant strains to daclizumab, pyrimethanil, cloketal, carbendazim and difenoconazole was determined. The results showed that there was significant difference in the sensitivity to the fungicides between the Botrytis cinerea strains and EC50 values between 0.0693 and 3.0578μg / mL with an average of 0.2045μg / mL. The EC50 values of three Botrytis cinerea strains were respectively EC50 7.97, 13.06 and 14.95 times respectively. There was a low correlation coefficient between the bacteriocid and the fungicide with different mechanisms of action. There was no cross-resistance. After 11 generations of subculture on drug-free PDA medium, the sensitivity of three relative resistant strains was significantly increased, indicating that the drug resistance can not be stably inherited.