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目的固有免疫受体(Toll like receptors,TLRs)在鼻咽癌发病过程中起重要作用,该研究检测固有免疫受体9(Toll like receptor-9,TLR9)在鼻咽癌癌组织与癌旁组织中的表达,探讨信号因子TLR9表达与鼻咽癌临床病理之间的联系。方法收集近1年97例鼻咽癌患者临床标本,同时收集所有病例的一般情况和临床病理特征等资料。应用RT-PCR、Western blot方法检测TLR9在鼻咽癌及癌旁组织中的基因和蛋白表达水平。采用免疫组化、免疫荧光等方法检测TLR9在鼻咽癌及癌旁组织中的分布规律,并结合临床参数探讨TLR9与鼻咽癌临床病理特征的相关性。结果 RT-PCR结果显示,与癌旁组织相比,TLR9 m RNA在鼻咽癌组织中表达明显增高,具有显著统计学意义(P<0.01);Western blot结果显示,与癌旁组织相比,TLR9在鼻咽癌组织中表达明显增高,具有显著统计学意义(P<0.01)。TLR9蛋白表达在鼻咽癌组织中的增高与鼻咽癌的部分临床参数如肿瘤分化程度、临床分期等密切相关。免疫组化显示TLR9在癌旁组织中表达呈弱阳性,而在鼻咽癌组织中表达明显增强,其主要分布于肿瘤细胞以及炎性细胞等部位。免疫荧光进一步提示HLA-DR阳性的炎症细胞为TLR9因子分泌的重要来源。结论 TLR9在鼻咽癌组织中表达明显增高,且与临床参数密切相关,说明TLR9可能在鼻咽癌病理形成过程中发挥一定作用,可能作为判断临床预后的重要指标。
Objective Toll-like receptors (TLRs) play an important role in the pathogenesis of nasopharyngeal carcinoma (NPC). This study examined the role of innate immune receptor 9 (TLR9) in the pathogenesis of nasopharyngeal carcinoma In order to explore the relationship between TLR9 expression and clinical pathology of NPC. Methods Ninety-seven patients with nasopharyngeal carcinoma in recent one year were collected, and the general conditions and clinical pathological characteristics of all cases were collected. The gene and protein expression of TLR9 in nasopharyngeal carcinoma and its adjacent tissues were detected by RT-PCR and Western blot. Immunohistochemistry and immunofluorescence were used to detect the distribution of TLR9 in nasopharyngeal carcinoma and its adjacent tissues. The correlation between TLR9 and the clinicopathological features of nasopharyngeal carcinoma was also discussed. Results RT-PCR results showed that the expression of TLR9 m RNA in nasopharyngeal carcinoma tissues was significantly higher than that in adjacent non-cancerous tissues (P <0.01). Western blot results showed that, compared with adjacent tissues, TLR9 expression in nasopharyngeal carcinoma was significantly increased, with significant statistical significance (P <0.01). The increase of TLR9 protein expression in nasopharyngeal carcinoma is closely related to some clinical parameters of NPC, such as the degree of tumor differentiation and clinical stage. Immunohistochemistry showed that TLR9 expression was weakly positive in paracancerous tissues and significantly increased in nasopharyngeal carcinoma tissues, which mainly distributed in tumor cells and inflammatory cells. Immunofluorescence further suggests that HLA-DR-positive inflammatory cells are an important source of TLR9 secretion. Conclusion The expression of TLR9 in nasopharyngeal carcinoma tissue was significantly increased, and closely related with clinical parameters, TLR9 may play a role in the pathogenesis of nasopharyngeal carcinoma, which may be used as an important indicator of clinical prognosis.