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目的建立测定榄香烯及榄香烯注射液含量测定中各组分不能完全得以分离的毛细管柱气相色谱法。方法采用毛细管柱气相色谱法,安捷伦6890型气相色谱仪;安捷伦DB-225毛细管柱,(50%氰丙基苯基)二甲基聚硅氧烷为固定液,长30m、内径0.32mm、膜厚0.25μm。程序升温100℃保持2min,以1℃/min速率升至140℃;载气:99.99%氮气,流速1mm/min;采用分流进样,进样量1μL。结果采用毛细管柱气相色谱法能较大地提高组分之间的分离度。榄香烯的线性关系为Y=9.55X-0.036(r=0.9991)。平均回收率为100.5%,相对标准偏差(RSD)为1.20%(n=9)。结论本方法准确,专属性好,可用作控制榄香烯及榄香烯注射液质量的方法。
OBJECTIVE To establish a capillary gas chromatographic method for the determination of elemene and elemene injection in the determination of the components can not be completely separated. Methods The capillary column gas chromatography with Agilent 6890 gas chromatograph, Agilent DB-225 capillary column with (50% cyanopropyl phenyl) dimethylpolysiloxane as stationary phase, length 30 m, inner diameter 0.32 mm, membrane Thick 0.25μm. The program temperature was maintained at 100 ℃ for 2min, and the temperature was raised to 140 ℃ at 1 ℃ / min. The carrier gas was 99.99% nitrogen and the flow rate was 1mm / min. The injection volume was 1μL. Results Capillary gas chromatography can greatly improve the separation between components. The linear relationship of elemene is Y = 9.55X-0.036 (r = 0.9991). The average recovery was 100.5% with a relative standard deviation (RSD) of 1.20% (n = 9). Conclusion This method is accurate and specific, and can be used as a method to control the quality of elemene and elemene injection.