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目的 从母血中分离胎儿细胞并确定其来自胎儿。方法 从孕早、中期各 2 0名、分娩后 15名的母血中富集并分离有核细胞。用 Y特异性探针 (PY3.4)行荧光原位杂交 ,从中识别胎儿细胞。结果 孕早、中期孕妇各怀 15名男胎。阳性细胞比例是 1∶ 6 5 2 8.0及 1∶ 2 783.8。与同期 10名女胎阳性细胞相比 ,差别有高度显著性。分娩 1周内的 3名 ,阳性率与孕中期的差别没有显著性。分娩 3个月后的阳性率与同期 10名女胎相比 ,差别无显著性。结论 提示在妊娠 5 0天就可以从母血中检测到胎儿细胞 ,并随孕期的进展而数量增加。分娩 1周后母血中仍有胎儿细胞存在 ,分娩 3个月后母血中未能检测到胎儿细胞。
Objective To isolate fetal cells from maternal blood and determine that it is from the fetus. Methods From 20 pregnant women in the early pregnancy to 20 pregnant women in the mid-term, 15 nuclear blood samples were collected for enrichment and separation of nucleated cells. Fetal cells were identified by fluorescence in situ hybridization with a Y-specific probe (PY3.4). Results Pregnancy early, mid-pregnant women each pregnant with 15 male fetuses. The positive cell ratios were 1: 6 5 2 8.0 and 1: 2 783.8. Compared with 10 female fetus positive cells in the same period, the difference was highly significant. 3 within 1 week of delivery, the positive rate and the second trimester difference was not significant. After 3 months of delivery the positive rate compared with 10 female fetuses at the same period, the difference was not significant. Conclusions suggest that fetal cells can be detected from maternal blood at 50 days of gestation and increase in number as the pregnancy progresses. There is still fetal cells in maternal blood after 1 week of delivery, and fetus cells can not be detected in maternal blood 3 months after delivery.