论文部分内容阅读
To characterize genes associated with differentiation/apoptosis induced by all- trans retinoic acid (ATRA) in human lung cancer cells, the mRNA differential display was employed and a cDNA fragment RAa7 was obtained which is up-regulated by ATRA. Search for homologous sequences in GenBank databases demonstrates that RAa7 is a novel sequence which has never been reported. Here evidence is provided that the RAa7 gene may be a tissue-specific expressing gene and has a major transcript of 1.4 kb. RAa7 gene is down-regulated during differentiation or apoptosis in several other kinds of lung cancer cells induced by ATRA and N- (4-hydroxyphenyl) retinamide (4HPR) . This suggests that either the up- or down-regulation of RAa7 gene may be involved in the processes of cell differentiation or apoptosis.
To characterize genes associated with differentiation/apoptosis induced by all- trans retinoic acid (ATRA) in human lung cancer cells, the mRNA differential display was employed and a cDNA fragment RAa7 was obtained which is up-regulated by ATRA. Search for homologous sequences in GenBank represents that RAa7 is a novel sequence which has never been reported. Here evidence is provided that the RAa7 gene may be a tissue-specific expressing gene and has a major transcript of 1.4 kb. RAa7 gene is down-regulated during differentiation or apoptosis In several other kinds of lung cancer cells induced by ATRA and N- (4-hydroxyphenyl) retinamide (4HPR) . This suggests that either the up- or down-regulation of RAa7 gene may be involved in the processes of cell differentiation or apoptosis.