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目的:研究旱生香茶菜的化学成分。方法:采用多种色谱技术和方法进行分离,通过波谱分析鉴定化合物结构。采用MTT法进行人体肿瘤细胞毒体外活性实验。结果:从旱生香茶菜叶中分离并鉴定了10个三萜类化合物:齐墩果酸(1),乌苏酸(2),maslinicacid(3),2α-羟基乌苏酸(4),hederagenin(5),3β,23-dihydroxy-urs-12-en-28-oicacid(6),arjunolicacid(7),asiaticacid(8),2α,3α,24-trihydroxyolean-12-en-28-oicacid(9),2α,3α,24-trihydroxyurs-12-en-28-oicacid(10)。测试了10个化合物对3种(K562,MKN45,HepG2)人体肿瘤细胞株的细胞毒活性。结论:化合物1-10均首次从该植物中分离得到,并讨论了它们的生源关系。所有三萜化合物对上述三种人体肿瘤细胞株都没有细胞毒活性。
Objective: To study the chemical constituents of xerophthalmus laevigata Methods: A variety of chromatographic techniques and methods were used for the separation and identification of compounds by spectroscopic analysis. MTT assay was used to determine the in vitro cytotoxic activity of human tumor cells. Results: Ten triterpenes were isolated and identified from the leaves of A. officinalis: oleanolic acid (1), ursolic acid (2), maslinicacid (3), 2α- (6), arjunolicacid (7), asiaticacid (8), 2α, 3α, 24-trihydroxyolean-12-en-28-oicacid (9), 2α, 3α, 24-trihydroxyurs-12-en-28-oicacid (10). Ten compounds were tested for cytotoxic activity against three human tumor cell lines (K562, MKN45, HepG2). CONCLUSION: Compounds 1-10 were isolated from this plant for the first time and discussed their biogenesis. All triterpene compounds have no cytotoxic activity against the above three human tumor cell lines.