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目的:建立茅苍术水溶性成分的HPLC指纹图谱并测定苍术苷A含量,为该药材的质量控制与资源开发提供参考。方法:利用HPLC建立茅苍术的指纹图谱并测定苍术苷A含量,流动相乙腈(A)-0.25%甲酸水溶液(B)梯度洗脱(0~17min,2%~5%A;17~25 min,5%~12%A;25~35 min,12%A;35~50 min,12%~17%A;50~70 min,17%~34%A;70~80min,34%~40%A),检测波长221 nm,流速0.6 m L·min-1。采用外标法测定苍术苷A在10批茅苍术药材中含量。结果:建立了茅苍术水溶性成分的HPLC指纹图谱,检出共有峰18个,精密度、稳定性、重复性试验中各共有峰相对保留时间和相对峰面积的RSD均<3%。10批茅苍术水提物中苍术苷A含量较稳定,平均质量分数2.775 mg·g-1。结论:HPLC指纹图谱方法与苍术苷A的含量测定方法准确、稳定、简便,可有效控制茅苍术药材的内在质量。
OBJECTIVE: To establish HPLC fingerprinting and to determine the content of atractyloside A in water-soluble fraction of Atractylodes lancea, providing references for the quality control and resource development of this herb. Methods: The fingerprint of Atractylodes macrocephala was established by HPLC and the content of atractyloside A was determined. The mobile phase was eluted with a gradient of acetonitrile-A.25% formic acid (B) (0-17 min, 2% -5% A, 17-25 min , 50% to 12% A, 25% to 35%, 12% A, 35% to 50%, 12% to 17% A, 50% to 70%, 17% to 34% A), detection wavelength 221 nm, flow rate 0.6 m L · min-1. The external standard method was used to determine the content of atractyloside A in 10 batches of Atractylodes. Results: The HPLC fingerprinting of water-soluble constituents of Atractylodes lancea was established. The average peak area (RSD) and the relative peak area of all the common peaks were all less than 3%. 10 Atractylodes Rhizoma water extract atractyloside A content is relatively stable, the average mass fraction of 2.775 mg · g-1. Conclusion: HPLC fingerprint method and atractyloside A content determination method is accurate, stable and simple, which can effectively control the intrinsic quality of Atractylodes.