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有关正常肝细胞体外培养的实验研究,国内外多介绍动物肝细胞的培养,而对人体正常肝细胞则仅见短期培养的报道。为了建立正常肝细胞株,为肝癌实验研究提供一个良好的体外模型,我们在建成人体肝癌等细胞系的基础上,尝试了正常肝细胞的培养。由于人体正常肝组织来源很少,不能满足肝细胞培养条件研究的需要,我们首先采用大白鼠、新生公牛肝及人的胚肝等材料,进行肝细胞体外培养的模拟实验。现将主要实验及结果简单小结如下。一、肝组织处理组织块用解剖刀切或眼科弯剪剪成小块;细胞悬液以0.15％胰蛋白酶(Difco,1∶250)消化或剪成糊状后以不锈钢网过滤制备。前者适用于各种组
For experimental studies on the in vitro culture of normal hepatocytes, the culture of liver cells in animals was introduced at home and abroad, but only short-term culture of normal human hepatocytes was reported. In order to establish a normal hepatocyte cell line and provide a good in vitro model for the experimental study of liver cancer, we have tried normal hepatocyte culture based on the establishment of human liver cancer cell lines. Due to the fact that human normal liver tissue has few sources and can not meet the needs of studies on the condition of hepatocyte culture, we first used rat, newborn bull liver and human embryo liver to carry out simulated experiments of hepatocyte culture in vitro. The main experiments and results are briefly summarized below. First, the liver tissue treated tissue blocks were cut into small pieces with a scalpel or ophthalmic curved scissors; cell suspensions were digested with 0.15% trypsin (Difco, 1:250) or cut into a paste and then filtered with a stainless steel mesh. The former applies to various groups