Objective. To investigate the cold preservation effect of rat livers by modified storage method with self-made HYD solution. Methods. The modified method was that the vascular bed of rat livers was expanded with an additional 20 to 40ml self-made HYD solution/100g liver. After removing the liver, the extra HYD solution expressed as % liver weight was entrapped via portal infusion by tying off the supra-and infra hepatic inferior vena cava. According to the amount of extra HYD solution, 40 rats were randomly divided into four groups including: control group with conventional storage method, 20% group, 30% group and 40% group. The preservation effect of modified storage method with that of conventional storage method by using isolated perfused rat liver model was compared. Results. Bile production and all the indices of hepatic microcirculation including portal perfusion pressure, en-dothelin-1 in the effluent, trypan blue distribution time and histology in modified method groups were significantly su-perior to those in control group ( P ＜ 0.05). The liver enzymes in 30% group were markedly lower than those in con-trol group (P＜ 0.05). The preservation effect of rat hver in 30% group was the best among the modified methodgroups. Conclusion. The modified cold storage method is effective and may have potential for clinical apphcation for hverpreservation.