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目的:探讨运用尿动力学方法研究cyclotraxin-B(CTX-B)抑制大鼠膀胱过度活动症(OAB)的可行性。方法:选择30只成年SD雌性大鼠,随机分为对照组、模型组和CTX-B组,腹腔单次注射环磷酰胺(200mg/kg)建立OAB大鼠模型。建模后第2天采用BL-410生物机能实验系统测定膀胱压参数,记录逼尿肌不稳定(DI)收缩频率、最大排尿压(MVP)、最大膀胱容量(MCC)和排尿间隔(ICI)。用光镜观察三组大鼠膀胱组织的病理学改变。结果:模型组和CTX-B组大鼠在充盈期均出现DI,形成锯齿状图型,DI收缩频率和MCC明显升高,MVP和ICI明显降低,其差异有显著统计学意义(P<0.01)。CTX-B组的膀胱病理学改变较模型组明显减轻。结论:CTX-B可显著抑制OAB的逼尿肌不稳定收缩。
Objective: To investigate the feasibility of using cyclotraxin-B (CTX-B) to inhibit overactive bladder in rats by urodynamic method. Methods: Thirty female adult SD rats were randomly divided into control group, model group and CTX-B group. OAB rat model was established by intraperitoneal injection of cyclophosphamide (200mg / kg). On the second day after modeling, BL-410 biomechanical system was used to measure the parameters of bladder pressure. The detrusor instability (DI) systolic frequency, maximal voiding pressure (MVP), maximal bladder volume (MCC) and micturition interval (ICI) . The pathological changes of the bladder tissue in the three groups were observed with light microscope. Results: The model group and the CTX-B group showed DI during filling, forming a jagged pattern. DI contraction frequency and MCC were significantly increased, while MVP and ICI were significantly decreased (P <0.01 ). CTX-B group of bladder pathological changes than the model group was significantly reduced. Conclusion: CTX-B can significantly inhibit the instability of detrusor contraction of OAB.