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目的:探讨氧自由基(OFR)和血栓素A2(TXA2)、前列环素(PGI2)在肺缺血再灌注损伤(IRI)中的作用以及三七总皂甙对其影响。方法:复制在体兔肺IRI模型。30只日本大耳兔,随机均分为三组:假手术组(S组)、缺血再灌注组(IR组)和缺血再灌注+三七总皂甙注射液组(PSN组)。检测血清超氧化物歧化酶(SOD)活性、黄嘌呤氧化酶(XO)活性和丙二醛(MDA)浓度,测定肺组织TXB2、6-keto-PGF1 α含量与比值的变化。结果:IR组血清XO、MDA均显著高于S组,SOD明显低于S组(均P<0．05和P<0．01)。 IR组肺组织TXB2显著高于S组(P<0．01),6-keto-PGF1α差异无明显;PNS组与IR组相比TXB2均下降非常明显(P<0．01),TXB2/6-keto-PGF1α降低很显著(P<0．01),与IR组相比差异有显著性(P<0．05和 P<0．01)。结论:三七总皂甙能降低氧自由基水平,抑制血小板释放TXA2,调控TXA2/PGI2的平衡,通过遏制无复流现象,减轻肺IRI。
Objective: To investigate the role of oxygen free radicals (OFR), thromboxane A2 (TXA2) and prostacyclin (PGI2) in lung ischemia-reperfusion injury (IRI) and the effects of Panax notoginseng saponins. METHODS: In vivo IRI models of rabbit lungs were reproduced. Thirty Japanese rabbits were randomly divided into three groups: sham operation group (S group), ischemia-reperfusion group (IR group), ischemia-reperfusion group and Panax notoginseng injection group (PSN group). Serum superoxide dismutase (SOD) activity, xanthine oxidase (XO) activity, and malondialdehyde (MDA) concentration were measured, and changes in TXB2, 6-keto-PGF1 α content and ratio were measured. Results: Serum XO and MDA levels in IR group were significantly higher than those in S group, and SOD was significantly lower than that in S group (all P<0.05 and P<0.01). TXB2 in lung tissue of IR group was significantly higher than that in S group (P<0.01), but there was no significant difference in 6-keto-PGF1α; TXB2 was significantly decreased in PNS group compared with IR group (P<0.01). TXB2/6 The decrease of keto-PGF1α was significant (P<0.01), and there was a significant difference compared with IR group (P<0.05 and P<0.01). Conclusion: Panax Notoginseng Saponins can reduce the level of oxygen free radicals, inhibit the release of TXA2 from platelets, regulate the balance of TXA2/PGI2, and reduce the IRI of lung by restraining the no-reflow phenomenon.