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目的:通过轮状病毒LLR株在牛肾细胞和Vero细胞中培养效果的比较,为轮状疫苗的生产筛选最佳的细胞基质和培养条件。方法:将轮状病毒LLR株按MOI 0.02分别接种牛肾细胞和Vero细胞,在相同培养条件下进行培养,每天观察两种细胞病变的情况,同时抽样检测病毒滴度,分析两种细胞对轮状病毒LLR株的敏感性。结果:牛肾细胞在感染轮状病毒LLR株后d 3病毒滴度达到最高,为6.8 lgCCID50.mL-1;而Vero细胞在感染轮状病毒LLR株后d 8病毒滴度达到最高,为7.3 lgCCID50.mL-1。轮状病毒LLR株在牛肾细胞和Vero细胞上均能达到满意的病毒表达量。结论:轮状病毒LLR株在Vero细胞中培养能够达到理想的病毒表达量,利用Vero细胞培养轮状病毒LLR株,有利于提高疫苗的产量和质量。
OBJECTIVE: To screen the best cell matrix and culture conditions for the production of rotavirus vaccine by comparing rotavirus LLR strains cultured in bovine kidney cells and Vero cells. Methods: Bovine kidney cells and Vero cells were inoculated with rotavirus strain LLR at a MOI of 0.02 and cultured under the same culture conditions. Two types of cytopathic effects were observed daily. The viral titers were also sampled and analyzed. Virulence of LLR strains. Results: The bovine kidney cells reached the highest d3 virus titer of 6.8 lg CCID50.mL-1 after infection with rotavirus LLR strain, while the highest d8 virus titer reached 7.3 in Vero cells after infection with rotavirus LLR strain lgCCID50.mL-1. The rotavirus LLR strain can achieve satisfactory viral expression levels on bovine kidney cells and Vero cells. Conclusion: The rotavirus strain LLR can be cultured in Vero cells to achieve the desired level of virus expression, and the use of Vero cells to culture rotavirus LLR strain can improve the yield and quality of vaccine.