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目的:探讨芝麻素对缺血再灌所致大鼠心肌损伤的保护作用及其可能机制。方法:SD大鼠50只随机分为正常对照组、模型组、假手术组、芝麻素低剂量组[80 mg/(kg·d)]及芝麻素高剂量组[160 mg/(kg·d)],每组10只。芝麻素组灌胃给予芝麻素7 d,每日1次,结扎冠状动脉左前降支建立心肌缺血再灌模型;假手术组大鼠只穿线,不接扎。HE染色观察心肌病理学变化,比色法测定血清肌酸激酶(CK)、乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)水平及心肌组织MDA、SOD水平。结果:芝麻素明显改善缺血再灌所致大鼠心肌损伤,减轻细胞水肿、变性、坏死及心肌间质炎症细胞浸润,降低血清CK、LDH水平(P<0.05或P<0.01)。芝麻素显著提高大鼠血清和心肌组织SOD活力,降低MDA水平(P<0.05或P<0.01),改善缺血再灌诱导的氧化应激。结论:芝麻素可明显改善缺血再灌所致大鼠心肌损伤,其机制与提高机体抗氧化能力、减轻缺血再灌所致氧化应激损伤有关。
Objective: To investigate the protective effect of sesamin on myocardial injury induced by ischemia-reperfusion in rats and its possible mechanism. Methods: Fifty SD rats were randomly divided into normal control group, model group, sham operation group, sesamin low dose group [80 mg / (kg · d)] and sesamin high dose group [160 mg / (kg · d )], Each group of 10. Sesamin was administered intragastrically for 7 days, and once a day. Ligation of left anterior descending coronary artery was used to establish model of myocardial ischemia and reperfusion. Rats in sham-operated group were only threaded and did not receive ligature. The pathological changes of myocardium were observed by HE staining. The contents of serum creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD) Level. Results: Sesamin obviously improved the myocardial injury induced by ischemia-reperfusion in rats, reduced the edema, degeneration, necrosis and infiltration of myocardial interstitial inflammatory cells and decreased the level of serum CK and LDH (P <0.05 or P <0.01). Sesamin increased the activity of SOD in serum and myocardium, reduced the level of MDA (P <0.05 or P <0.01), and improved the oxidative stress induced by ischemia reperfusion. Conclusion: Sesamin can significantly improve the myocardial injury caused by ischemia-reperfusion in rats, and its mechanism is related to the improvement of antioxidant capacity and the impairment of oxidative stress induced by ischemia-reperfusion.