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目的:探讨跑台运动对幼龄大鼠海马齿状回神经再生的影响。方法:选取20只5周龄SD大鼠,将其随机分为1周对照组、1周运动组、2周对照组和2周运动组。运动组大鼠以小强度分别进行为期1周和2周的跑台运动。实验后,大鼠腹腔注射溴脱氧尿嘧啶核苷(bromod-eoxyuridine,BrdU),使用免疫组织化学方法检测分析大鼠脑内海马齿状回(dentate gyrus,DG)和脑部扣带回(cingulate gyrus,CG)神经细胞的再生情况。结果:各组大鼠脑部海马齿状回和脑部扣带回均出现BrdU标记的阳性细胞,说明此区有新的神经细胞生成;其中1周运动组大鼠齿状回中的BrdU阳性细胞数显著多于1周对照组(P<0·05);2周运动组大鼠海马齿状回中的BrdU阳性细胞数与2周对照组相比无显著性差异(P>0·05)。此外,两个运动组大鼠脑部扣带回的BrdU阳性细胞数与相应的对照组之间无显著性差异(P>0·05)。结论:1周小强度跑台运动可促进幼龄大鼠脑部海马齿状回区的神经再生。
Objective: To investigate the effect of treadmill exercise on the regeneration of dentate gyrus in young rats. Methods: Twenty SD rats of 5 weeks old were selected and randomly divided into 1 week control group, 1 week exercise group, 2 weeks control group and 2 weeks exercise group. Exercise group rats with a small intensity for 1 week and 2 weeks of treadmill exercise. After the experiment, bromododeoxyuridine (BrdU) was injected intraperitoneally in rats. Immunohistochemistry was used to detect dentate gyrus (DG) and cingulate gyrus, CG) regeneration of nerve cells. Results: BrdU positive cells appeared in the hippocampal dentate gyrus and brain cortex in each group, which indicated that there were new neurons in this area. BrdU positive in dentate gyrus of rats in 1 week exercise group (P <0.05). The number of BrdU positive cells in hippocampal dentate gyrus in 2-week exercise group was not significantly different from that in 2-week control group (P> 0.05) ). In addition, there was no significant difference in the number of BrdU-positive cells in the brains between the two exercise groups and the corresponding control group (P> 0.05). Conclusion: One-week low-intensity treadmill exercise can promote the regeneration of hippocampal dentate gyrus in young rats.